期刊:Ecotoxicology and environmental safety ISSN:1090-2414 , 年:2018 . 卷:147   页码:455-460

原文链接:http://doi.org/10.1016/j.ecoenv.2017.08.061

摘要

Manure application contributes to the increased environmental burden of antibiotic resistance genes (ARGs). We investigated the response of tetracycline (tet) resistance genes and bacterial taxa to manure application amended with tetracyclines over two months. Representative tetracyclines (oxytetracycline, chlorotetracycline and doxycycline), tet resistance genes (tet(M), tet(O), tet(W), tet(S), tet(Q) and tet(X)) and bacterial taxa in the untreated soil, +manure, and +manure+tetracyclines groups were analyzed. The abundances of all tet resistance genes in the +manure group were significantly higher than those in the untreated soil group on day 1. The abundances of all tet resistance genes (except tet(Q) and tet(X)) were significantly lower in the +manure group than those in the +manure+tetracyclines group on day 30 and 60. The dissipation rates were higher in the +manure group than those in the +manure+tetracyclines group. Disturbance of soil bacterial community composition imposed by tetracyclines was also observed. The results indicated that tetracyclines slowed down the dissipation of tet resistance genes in arable soil after manure application. Application of manure amended with tetracyclines may provide a significant selective advantage for species affiliated to the taxonomical families of Micromonosporaceae, Propionibacteriaceae, Streptomycetaceae, Nitrospiraceae and Clostridiaceae. Copyright 漏 2017 Elsevier Inc. All rights reserved.

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关键词
Antibiotic resistance genes;  Manure application;  Soil bacteria;  Tetracycline resistance genes;  Tetracyclines

作者信息

作者机构:
     [1] National Laboratory of Safety Evaluation (Environmental Assessment) of Veterinary Drugs and the Guangdong Provincial Key Laboratory of Veterinary Drugs Development and Safety Evaluation, South China Agricultural University, 483 Wushan Road, Guangzhou 510642, China.
     [2] College of Natural Resources and Environment, South China Agricultural University, 483 Wushan Road, Guangzhou 510642, China.
     [3] National Laboratory of Safety Evaluation (Environmental Assessment) of Veterinary Drugs and the Guangdong Provincial Key Laboratory of Veterinary Drugs Development and Safety Evaluation, South China Agricultural University, 483 Wushan Road, Guangzhou 510642, China. Electronic address: sunyx@scau.edu.cn.
     [4] National Laboratory of Safety Evaluation (Environmental Assessment) of Veterinary Drugs and the Guangdong Provincial Key Laboratory of Veterinary Drugs Development and Safety Evaluation, South China Agricultural University, 483 Wushan Road, Guangzhou 510642, China. Electronic address: zlzeng@scau.edu.cn.